Journal: The Plant Journal
Article Title: Berberine bridge enzyme‐like oxidases orchestrate homeostasis and signaling of oligogalacturonides in defense and upon mechanical damage
doi: 10.1111/tpj.70150
Figure Lengend Snippet: OGOX1::GUS transgenic leaves show increased GUS activity in response to pathogens, wounding and elicitor treatment. GUS activity was analyzed in OGOX1::GUS # 3.2 line. Results with a second independent line (#2.3) are in Figure . (a) Rosette leaves were drop‐inoculated with B. cinerea conidia (5 μl, ×10 5 spore ml −1 ) or PDB (mock) as a control and GUS assay was performed 48 h post‐inoculation. (b) Rosette leaves were infiltrated with P. syringae pv. tomato DC3000 at OD = 0.002 or with H 2 O (mock‐infiltrated). GUS assay was performed 72 hpi. (c) Rosette leaves were drop‐inoculated at punctured sites with P. carotovorum cells (5 μl, OD 600 = 0.025) or 50 m m potassium phosphate buffer pH 7.0 (mock‐inoculated). GUS assay was performed 14 hpi. (d) Untreated excised leaves analyzed at 72 h as a control. (e) Rosette leaves were wounded by crushing with knurled tweezers; GUS assay was performed 1 h after crushing. (f) Rosette leaves were infiltrated with OGs (200 μg ml −1 ), flg22 (100 n m ), and water as control. GUS assay was performed 1‐h post‐infiltration.
Article Snippet: Histochemical staining for GUS activity was performed by incubating leaves in the staining buffer (0.5 mg ml −1 X‐Glca [5‐bromo‐4‐chloro‐3‐indolyl β‐ d ‐glucuronic acid cyclohexylammonium salt; Duchefa Biochemie]; 2 m m K 3 [Fe(CN) 6 ]; 2 m m K 4 [Fe(CN) 6 ]; 0.2% Triton X‐100; 50 m m buffer sodium phosphate, pH 7.2; 2% DMSO) overnight at 37°C, with shaking.
Techniques: Transgenic Assay, Activity Assay, Control, GUS Gene Assay
